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Production of monoclonal antibodies against Xanthomonas campestris pv. mangiferaeindicae and their use to investigate differences in virulence
Author(s) -
Sanders Gina M.,
Verschoor J.A.,
Wyngaard S.,
Korsten L.,
Kotzé J.M.
Publication year - 1994
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1994.tb04395.x
Subject(s) - xanthomonas campestris , virulence , monoclonal antibody , microbiology and biotechnology , biology , xanthomonas , virology , antibody , bacteria , genetics , gene
G.M. SANDERS, J.A. VERSCHOOR, S. VAN WYNGAARD, L. KORSTEN AND J.M. KOTZE 1994. Four Xanthomonas campestris pv. mangiferaeindicae isolates from mango black spot lesions were grouped according to differences in virulence and used to raise monoclonal antibodies (mAbs). Two immunization approaches were followed. In the first, four groups of mice were immunized, each with a different isolate and the spleens from each group homogenized together for cell fusion. The second approach entailed immunization of a single group of mice with bacteria pooled from all four isolates. The resultant mAbs were characterized with regard to the antigen binding specificity and antibody class. A relationship between mAb binding specificity and virulence of the bacteria was shown by Western blot analysis.