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Detection of low levels of specific Salmonella species by fluorescent antibodies and flow cytometry
Author(s) -
McClelland Rosemary G.,
Pinder A.C.
Publication year - 1994
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1994.tb03447.x
Subject(s) - flow cytometry , salmonella , monoclonal antibody , antibody , serotype , fluorescence , microbiology and biotechnology , biology , chemistry , bacteria , immunology , genetics , physics , quantum mechanics
The use of fluorescently‐labelled monoclonal antibodies, with detection by multi‐parameter flow cytometry, was investigated for the rapid detection of salmonellas in pure cultures. Accurate detection of specific Salmonella serotypes was demonstrated down to levels of below 10 4 cells ml ‐1 (within 30 min) and 1 cell ml ‐1 (after 6 h non‐selective pre‐enrichment). This level of sensitivity was attainedeven in the presence of high levels of other bacterial species that would otherwise have interfered with the results. With combinations of different antibodies, each with a unique fluorescent label, simultaneous analysis for two species was possible.