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Production and viability of coccoid forms of Campylobacter jejuni
Author(s) -
Boucher S.N.,
Slater E.R.,
Chamberlain A.H.L.,
Adams M.R.
Publication year - 1994
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1994.tb03078.x
Subject(s) - campylobacter jejuni , microbiology and biotechnology , campylobacter , biology , bacteria , genetics
Studies were conducted into the formation and physiological state of coccoid cells of a strain of the human and animal pathogen Campylobacter jejuni. It was found that growth phase and the presence of chloramphenicol did not affect the rate of shape transformation from spiral to coccoid, while nutrient limitation, aeration of the medium and the presence of free‐radical scavengers had profound effects. Coccoid cells were found to reduce the tetrazolium salts INT (2‐( p ‐iodophenyl)‐3‐( p ‐nitrophenyl)‐5‐phenyl tetrazolium chloride) and CTC (5‐cyano‐2,3‐ditolyl tetrazolium chloride) to their respective formazans and this was linked to cellular respiration. However, respiring coccoid cells could not sustain their existence in prolonged adverse conditions, and it was concluded that they represent a degenerative stage rather than a dormant state of the organism.