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Thermostability of bacterial luciferase expressed in different microbes
Author(s) -
Mackey B.M.,
Cross D.,
Park S.F.
Publication year - 1994
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1994.tb03058.x
Subject(s) - luciferase , thermostability , listeria monocytogenes , vibrio harveyi , plasmid , biology , microbiology and biotechnology , escherichia coli , vibrio , reporter gene , bioreporter , intracellular , bacteria , biochemistry , chemistry , gene , gene expression , transfection , genetics , enzyme
Bacterial luciferase was used to investigate the relationship between the thermostability of a cytoplasmic reporter molecule and cellular heat resistance. The luciferase activity of Vibrio fischeri was expressed in strains of Escherichia coli, Salmonella typhimurium, Listeria monocytogenes and Brochothrix thermosphacta following transformation with plasmid pSP13 carrying the luxAB genes. The thermostability of intracellular luciferase varied depending on the organism in which it was expressed, but was not related to the cellular heat resistance of the different organisms. Addition of xylitol to the heating medium protected against loss of viability and inactivation of intracellular luciferase. Glycerol also protected against loss of viability but was less effective at preventing thermal denaturation of luciferase.