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Identification of Campylobacter jejuni, Campylobacter coli and Campylobacter lari by the nucleic acid amplification system NASB R
Author(s) -
Uyttendaele M.,
Schukkink R.,
Gemen B.,
Debevere J.
Publication year - 1994
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1994.tb02821.x
Subject(s) - campylobacter jejuni , campylobacter , campylobacter coli , microbiology and biotechnology , nucleic acid , identification (biology) , biology , chemistry , bacteria , genetics , botany
M. UYTTENDAELE, R. SCHUKKINK, B. VAN GEMEN AND J. DEBEVERE. 1994. NASBA R , an isothermal amplification technique for nucleic acids, was evaluated for the specific identification of Campylobacter jejuni, Camp. coli and Camp. lari. A set of primers and a probe were chosen from the 16S rRNA sequence of Campylobacter. The probe was hybridized in solution with the amplified nucleic acids of 12 Campylobacter species and nine other Gram‐negative bacteria. The probe was shown to hybridize specifically to the amplified single‐stranded RNA of Camp. jejuni, Camp. coli and Camp. lari in an enzyme‐linked gel assay (ELGA). In a Camp. jejuni model system the combination of NASBA R and ELGA was able to detect ca 1000 rRNA molecules. The presence of an excess of Gram‐negative bacteria did not influence the sensitivity of detection. A number of 6 cfu of Camp. jejuni , present in a total count of 4 times 10 6 cfu of Gram‐negative bacteria, resulted in a positive hybridization signal.