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The effect of electron beam irradiation and modified pH on the survival and recovery of Escherichia coli
Author(s) -
Flelding L.M.,
Cook P.E.,
Grandison A.S.
Publication year - 1994
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1994.tb01648.x
Subject(s) - irradiation , escherichia coli , chemistry , nuclear chemistry , radiochemistry , biochemistry , physics , nuclear physics , gene
The severity of radiation processing can be reduced by combining irradiation with other treatments, such as low pH. An exponential phase culture of Escherichia coli was irradiated at doses of 0–2.4 kGy at pH values ranging between 7.0 and 4.0, in an enriched nutrient broth. At pH 4.3 and above there was no significant effect of lowering the pH prior to irradiation. At pH 4.13 and 4.0, a much higher level of cell death occurred compared with irradiation at pH 7.0. This synergistic effect was observed only when the pH was lowered before radiation processing.