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Effect of substrate on the production of antifungal volatiles from Bacillus subtilis
Author(s) -
Fiddaman P.J.,
Rossall S.
Publication year - 1994
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1994.tb01646.x
Subject(s) - bacillus subtilis , substrate (aquarium) , food science , chemistry , agar , antifungal , microbiology and biotechnology , nutrient agar , bacterial growth , agar plate , biology , bacteria , biochemistry , ecology , genetics
An antibiotic‐producing strain of Bacillus subtilis has been shown to produce potent antifungal volatiles (AFV). These volatiles are active against a range of fungal species and are produced on a range of growth media and in loam‐based compost. In vitro antifungal volatile activity on nutrient agar is enhanced with the addition of D‐glucose, complex carbohydrates and peptones. The addition of L‐glucose led to significantly less AFV activity than comparable levels of D‐glucose. Growth studies in liquid culture revealed that B. subtilis failed to grow in response to L‐glucose. Further growth studies on solid media showed no clear correlation between enhanced bacterial growth and increases in in vitro AFV activity in response to supply of substrates. Low level AFV activity was also detected from oilseed rape roots inoculated with B. subtilis . Gas chromatography mass spectrometry headspace analysis of B. subtilis cultures grown on various substrates revealed common similarities between substrates promoting AFV activity, although it was not possible to isolate individual antifungal compounds.