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Thermal resistance of partially purified proteinase of Pseudomonas fluorescens P‐26
Author(s) -
Uplacksh V.K.,
Mathur D.K.,
Malik R.K.
Publication year - 1994
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1994.tb01640.x
Subject(s) - pseudomonas fluorescens , skimmed milk , fractionation , chemistry , d value , chromatography , saturation (graph theory) , phosphate buffered saline , pseudomonas , food science , bacteria , biology , mathematics , genetics , combinatorics
Heat resistance of the Pseudomonas fluorescens P‐26 proteinase in terms of D ‐value was studied in whole milk, skim milk, whey and 0.05 mol 1 ‐1 phosphate buffer at 72.5, 130, 135, 140, 145 and 150°C subsequent to its partial purification through (NH 4 ) 2 SO 4 precipitation (45–65% saturation) and solvent fractionation with 1.0 to 2.0 volumes of isopropanol. The D ‐value was maximum for the proteinase at all temperatures when determined in whole milk ( D 150 = 0.088).