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Effect of some factors on determination of viable microbial cells by peroxyoxalate chemiluminescence method
Author(s) -
Oh Sang,
Cha Seung
Publication year - 1994
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1994.tb01628.x
Subject(s) - menadione , chemistry , chemiluminescence , enterobacter aerogenes , escherichia coli , serratia marcescens , nuclear chemistry , enterobacter cloacae , chromatography , tris , biochemistry , enterobacteriaceae , gene , enzyme
The effects of physical and chemical factors on the production of H 2 O 2 from Escherichia coli cells were studied. When 20 mmol 1 ‐1 Tris‐HCl buffer was used for this purpose the electron transport system (ETS) showed the highest activity at pH 7.6‐8.2. KCN promoted the production of H 2 O 2 from E. coli cells, and the optimum concentration was changed in different reaction times and pH values. Glucose, 5 mg ml ‐1 , increased the ETS activity about twofold. The other substrates and surfactants did not increase the chemiluminescence intensity. NaNO 2 and Na 2 SO 4 in inorganic salts significantly reduced the ETS activity above 70%. In addition, the optimum temperature for the production of H 2 O 2 was 30°C in this study. When glucose (5 mg ml ‐1 ) and KCN (0.2 mmol 1 ‐1 ) were added to the reaction buffer containing 0.5 mmol 1 ‐1 menadione, the detectable minimum cell densities (averages of triplicate assay) of E. coli, Enterobacter cloacae and Serratia marcescens were 5 times 10 3 cells ml ‐1 , 10 4 cells ml ‐1 and 10 4 cells ml ‐1 respectively.