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Substrate affinity from bacterial strains and distribution water biofilms
Author(s) -
Frías J.,
Ribas F.,
Lucena F.
Publication year - 1994
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1994.tb01614.x
Subject(s) - water column , chemistry , biofilm , saturation (graph theory) , kinetics , substrate (aquarium) , enzyme kinetics , dissolved organic carbon , steady state (chemistry) , chromatography , michaelis–menten kinetics , environmental chemistry , bacteria , enzyme , enzyme assay , biochemistry , biology , ecology , genetics , physics , mathematics , combinatorics , quantum mechanics , active site
Biodegradable dissolved organic carbon (BDOC) is almost the only limiting factor of bacterial growth in drinking water. Two representative bacterial strains were isolated from a two‐column reactor for the determination of BDOC (from respectively the first and the second column) and used to estimate their growth kinetics in two different types of water: finished water from a water treatment plant and water collected at the outlet of the first column of the BDOC reactor. K s , the substrate saturation constant, was calculated by analogy with Michaelis‐Menten enzyme kinetics, and for each strain a lower K s value was observed for the type of water that it normally receives. Ks for in situ biofilms, from both first and second column and the same types of water, were also calculated according to a technique which uses a curve‐matching method with kinetic results from the non‐steady‐state experiments. In this case, the results were similar for the first and second column biofilms. However, a higher affinity was observed for finished water than for the water from the outlet of the first column in both biofilms.