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Characteristics of ‘atypical’, cytochrome oxidase‐negative Aeromonas salmonicida isolated from ulcerated flounders ( Platichthys flesus (L.))
Author(s) -
Wiklund T.,
Dalsgaard I.,
Eerola E.,
Olivier G.
Publication year - 1994
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1994.tb01110.x
Subject(s) - aeromonas salmonicida , platichthys , biology , flounder , microbiology and biotechnology , oyster , cytochrome , bacteria , oxidase test , fish <actinopterygii> , biochemistry , genetics , fishery , enzyme
‘Atypical’, cytochrome oxidase‐negative variants of the fish pathogen Aeromonas salmonicida , isolated from ulcerated flounder ( Platichthys flesus ), were studied using different methods. Two of the strains possessed a protein that corresponded to the A‐layer protein of Aer. salmonicida . The strains reacted with antibodies against the A‐layer and monoclonal antibodies against the O‐antigen of typical Aer. salmonicida . These tests confirm that the isolates from flounder should be classified as Aer. salmonicida . Analysis of the fatty acids showed that the isolates were rather homogenous but the values of the guanine plus cytosine content of the DNA of the bacteria varied too much for any conclusion to be drawn on their taxonomic location. The strains examined exhibited several biochemical characters that differed from those of the type strains of Aer. salmonicida subsp. salmonicida and Aer. salmonicida , subsp. achromogenes . The results suggest that these ‘atypical’, cytochrome oxidase‐negative variants may form a new subspecies of Aer. salmonicida .