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The effects of transgalactosylated oligosaccharides on gut flora metabolism in rats associated with a human faecal microflora
Author(s) -
Rowland I.R.,
Tanaka R.
Publication year - 1993
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1993.tb05201.x
Subject(s) - flora (microbiology) , microbiology and biotechnology , gut microflora , gut flora , microbial metabolism , biology , metabolism , bacteria , feces , biochemistry , genetics
The effect on various caecal bacteria and their metabolic activities of feeding diet containing transgalactosylated oligosaccharides (TOS) with or without Bifidobacterium breve (administered in the drinking water) was investigated in rats colonized with a human faecal microflora. TOS (5% w/w in diet) or TOS plus B. breve, given for 4 weeks, induced increases in caecal concentration of total anaerobic bacteria, lactobacilli and bifidobacteria, and decreases in numbers of enterobacteria. Caecal pH was significantly reduced by feeding TOS, as were the activities of β‐glucuronidase and nitrate reductase. In contrast, β‐glucosidase activity was increased in TOS‐fed rats. Dietary TOS was also associated with decreased conversion, by caecal contents, of the dietary carcinogen 2‐amino‐3‐methyl‐3H‐imidazo[4, 5‐ f ] quinoline (IQ) to its genotoxic 7‐hydroxy derivative.