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Role of haemagglutinins in adhesion of Erwinia carotovora to potato tissue
Author(s) -
Wallace Anne,
Pérombelon M.C.M.
Publication year - 1993
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1993.tb05191.x
Subject(s) - erwinia , adhesion , nutrient agar , microbiology and biotechnology , bacteria , agar , biology , strain (injury) , mannose , chemistry , biochemistry , genetics , organic chemistry , anatomy
The adhesion of two strains each of Erwinia carotovora subsp. carotovora and Erwinia carotovora subsp. atroseptica to potato tuber discs, leaflets and tuber cell cultures was examined and found to occur independently of the presence of either mannose‐sensitive (MSHA) or mannose‐resistant (MRHA) haemagglutinins. Adhesion was generally greater when bacteria were grown in nutrient broth than on phosphate‐buffered agar. The specific MSHA inhibitor α‐methyl mannoside reduced the adhesion of two strains to tuber discs and leaflets and the specific MRHA inhibitor, asialofetuin inhibited strains only on leaflets. A reduction in adhesion of a MSHA‐producing strain by α‐methyl mannoside was observed by scanning electron microscopy which found that adhesion was localized at intercellular junctions.