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A simple, rapid and sensitive presence/absence detection test for bacteriophage in drinking water
Author(s) -
Armon R.,
Kott Y.
Publication year - 1993
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1993.tb05159.x
Subject(s) - bacteriophage , bacteroides fragilis , escherichia coli , microbiology and biotechnology , bacteria , biology , agar , agar plate , chemistry , chromatography , antibiotics , biochemistry , genetics , gene
R. ARMON AND Y. KOTT. 1993. A rapid, simple and sensitive direct bacteriophage presence detection method for 500 ml drinking water samples has been developed. The method includes a glass device consisting of a jar containing the water sample and an immersible probe filled with solidified soft agar containing bacterial host cells. Host bacteria in logarithmic phase were added to the experimental volume and the probe was submerged. The entire device was incubated in a water bath at 36d̀C. Plaques of somatic bacteriophage infecting Escherichia coli strain CN 13 , could be detected within 3 h. Male‐specific bacteriophages infecting E. coli F+ amp were detected within 6 h. Bacteriophage infecting the anaerobe Bacteroides fragilis subsp. fragilis HSP40 were detected after 8 h. Application of this device and the associated technique, enabled a one‐step detection of 1 pfu of E. coli or Bact. fragilis specific bacteriophage in 500 ml drinking water samples.