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Time‐resolved fluoroimmunoassay as a method for detection of Erwinia chrysanthemi in potato peel extracts
Author(s) -
Wolf J.M.
Publication year - 1993
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1993.tb05140.x
Subject(s) - chromatography , chemistry , detection limit , erwinia , dilution , biochemistry , gene , physics , thermodynamics
J.M. VAN DER WOLF. 1993. Time‐resolved fluoroimmunoassay (TR‐FIA) was compared with double antibody sandwich (DAS)‐ELISA for detection of Erwinia chrysanthemi antigens in potato peel extracts. Pure cultures were used to optimize TR‐FIA with respect to the microplate washing procedure and dilution buffer compositions. The detection threshold level for spiked potato peel extracts with the optimized TR‐FIA format was 10 5 cells ml ‐1 as for the detection level of DAS‐ELISA. The signal to background ratios at concentrations above 10 5 cells ml ‐1 were higher with TR‐FIA than with DAS‐ELISA. The dynamic range of TR‐FIA was also superior to that of DAS‐ELISA. It can be concluded that TR‐FIA is an attractive alternative to DAS‐ELISA as a detection method for Erw. chrysanthemi, especially when quantification is required.