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A new method for the concentration of Cryptosporidium oocysts from water
Author(s) -
Vesey G.,
Slade J.S.,
Byrne M.,
Shepherd K.,
Fricker C.R.
Publication year - 1993
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1993.tb03412.x
Subject(s) - calcium carbonate , tap water , cryptosporidium , sodium bicarbonate , calcium , sodium carbonate , precipitation , chemistry , sodium hydroxide , sodium , bicarbonate , carbonate , calcium hydroxide , centrifugation , nuclear chemistry , chromatography , mineralogy , microbiology and biotechnology , environmental engineering , environmental science , biology , physics , organic chemistry , feces , meteorology
A novel method for the concentration of Cryptosporidium oocysts from water has been developed, based upon the precipitation of calcium carbonate. A 10 1 water sample is treated by adding solutions of calcium chloride and sodium bicarbonate and raising the pH value to 10 with sodium hydroxide. Crystals of calcium carbonate form and enmesh particles in the Cryptosporidium oocyst size range. The crystals are allowed to settle, the supernatant fluid is discarded and the calcium carbonate precipitate dissolved in sulphamic acid. The sample can be concentrated further by centrifugation. Recoveries of oocysts from seeded samples of deionized, tap and river water were in excess of 68%.