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Production and survival during storage of spray‐dried Bradyrhizobium japonicum cell concentrates
Author(s) -
Mary P.,
Moschetto N.,
Tailliez R.
Publication year - 1993
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1993.tb03035.x
Subject(s) - bradyrhizobium japonicum , spray drying , sucrose , chemistry , trehalose , food science , skimmed milk , biology , biochemistry , chromatography , bacteria , rhizobiaceae , genetics , symbiosis
Production of Bradyrhizobium japonicum cell concentrates by spray‐drying in skim milk plus sucrose medium and the feasability of storing dried inocula over long periods were investigated. Storage of spray‐dried cells under mild vacuum was equivalent to storage under nitrogen. Oxygen and ambient temperature were found detrimental for survival of dried cells. High initial cell concentration and storage under low relative humidities (< 23% RH) at 4°C increased the longevity of the inocula (> 10 9 cfu g ‐1 during at least a 25 week storage period) without altering the symbiotic properties of B. japonicum.