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Detection of Aeromonas hydrophila in food with an enzyme‐linked immunosorbent assay
Author(s) -
Merino Susana,
Camprubì Silvia,
Tomás J.M.
Publication year - 1993
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1993.tb03008.x
Subject(s) - aeromonas hydrophila , serotype , antibody , immunoassay , microbiology and biotechnology , aeromonas , virulence , biology , vibrionaceae , bacteria , immunology , biochemistry , gene , genetics
A microtitration plate, antibody capture, enzyme‐linked immunosorbent assay was developed for the detection of Aeromonas hydrophila serotype O : 11 (highly virulent strains). The assay utilizes a detector antibody which shows no cross‐reactions with Aeromonas strains other than serotype O : 11 or non‐ Aeromonas competing organisms. The detector antibody is mixed with the sample and incubated for 1 h, microcentrifuged and the supernatant fluid (unadsorbed antibody) titred in a microtitre plate coated with A. hydrophila cells from serotype O : 11. All the A. hydrophila strains from serotype O : 11 tested reacted strongly with the detector antibody. Also by culturing and performing the immunoassay with the detector antibody we established and quantified the presence of A. hydrophila O : 11 in different foods.