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Identification and quantitative analysis of degradation products of chlorhexidine with chlorhexidine‐resistant bacteria with three‐dimensional high performance liquid chromatography
Author(s) -
Ogase H.,
Nagai I.,
Kameda K.,
Kume S.,
Ono S.
Publication year - 1992
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1992.tb04972.x
Subject(s) - chlorhexidine , high performance liquid chromatography , chemistry , chromatography , degradation (telecommunications) , phenol , pyrogallol , bacteria , microbiology and biotechnology , organic chemistry , biology , dentistry , medicine , telecommunications , computer science , genetics
The products of the degradation of chlorhexidine by two chlorhexidine‐resistant strains of Achromobacter xylosoxidans , isolated from an ultrasonic hand washer, were identified by three‐dimensional HPLC. In the degradation process a pathway forming p ‐chlorophenol, probably through p ‐chloroaniline, was also observed, as was a pathway forming phenol and pyrogallol. The quantitative measurement of these products at their maximum absorption spectra by two‐dimensional HPLC suggested the presence of chlorhexidine‐degrading enzymes.