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A note on the use of a plasmid as a DNA probe in the detection of a Lactobacillus fermentum strain in porcine stomach contents
Author(s) -
Tannock G.W.,
McConnell Michelle A.,
Fuller R.
Publication year - 1992
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1992.tb04969.x
Subject(s) - lactobacillus fermentum , plasmid , strain (injury) , population , biology , microbiology and biotechnology , dna , lactobacillus , bacteria , genetics , lactic acid , medicine , anatomy , environmental health , lactobacillus plantarum
A plasmid (about 50 kb) was used as a DNA probe to enumerate, by colony hybridization, a strain of Lactobacillus fermentum in the stomach contents of eight piglets. The population sizes obtained by colony hybridization were in agreement with estimated levels calculated on the basis of plasmid profiling of colonies isolated at random from the total lactobacillus population.