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A combined PCR and selective enrichment method for rapid detection of Listeria monocytogenes
Author(s) -
Fitter S.,
Heuzenroeder M.,
Thomas C.J.
Publication year - 1992
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1992.tb04968.x
Subject(s) - listeria monocytogenes , polymerase chain reaction , biology , microbiology and biotechnology , serial dilution , enrichment culture , listeria , oligonucleotide , oligomer restriction , real time polymerase chain reaction , bacteria , gene , genetics , pathology , medicine , alternative medicine
Development of a routine detection assay for Listeria monocytogenes in foods that uses the polymerase chain reaction (PCR) and enrichment cultures was investigated. Oligonucleotide primers were chosen to amplify a 3’region of L. monocytogenes hly A gene spanning a conserved Hin dIII site. PCR detection sensitivity for L. monocytogenes in dilutions of pure enrichment cultures was between 50 and 500 colony forming units. A short enrichment period before PCR amplification allowed detection of the organisms in a range of complex foods contaminated with 10 4 cfu/g. Detection sensitivity for the assay in the presence of chicken skin and soft cheese was determined at 10–100 cfu/g. Utilization of enrichment cultures and PCR allowed identification of the organism within 24 h or 2 days.