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Optimization of an amplified system for the detection of Clostridium tyrobutyricum on nitrocellulose filters by use of monoclonal antibody in a gelified medium
Author(s) -
Nedellec M.,
Cleret J.J.,
Robreau G.,
Talbot F.,
Malcoste R.
Publication year - 1992
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1992.tb04879.x
Subject(s) - nitrocellulose , monoclonal antibody , membrane , antibody , collodion , bacteria , chromatography , chemistry , microbiology and biotechnology , biology , biochemistry , immunology , genetics
A method of immunological detection by an amplified system has been applied to detect Clostridium tyrobutyricum on nitrocellulose membranes. This method, adapted from an amplification technique, was used in a gelified medium with a monoclonal antibody raised against Cl. tyrobutyricum and an alkaline phosphatase‐conjugated antibody to mouse IgM. The technique permits the detection of about 30 bacteria per spot on membranes.