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Lack of colonization of 1 day old chicks by viable, non‐culturable Campylobacter jejuni
Author(s) -
Mederma G.J.,
Schets F.M.,
Giessen A.W.,
Havelaar A.H.
Publication year - 1992
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1992.tb01868.x
Subject(s) - campylobacter jejuni , biology , microbiology and biotechnology , incubation , campylobacter , embryonated , colonization , viable count , feces , bacteria , inoculation , immunology , biochemistry , genetics
G.J. MEDEMA, F.M. SCHETS, A.W. VAN DE GIESSEN AND A.H. HAVELAAR. 1992. Seven strains of Campylobacter jejuni , isolated from various sources [human ( n = 2), chicken ( n = 3), water ( n = 2)], were studied under starvation conditions in filter‐sterilized and pasteurized surface water by acridine orange direct count (AODC), viable count (DVC) and culture methods. Plate counts showed a rapid decline (2 log‐units/day) for all strains under these conditions. Only one of the seven strains (14%) showed a (prolonged) viable, non‐culturable ‘state’. The ability of these viable, non‐culturable cells to colonize the intestine was tested on day‐old chicks. The infectious oral dose of freshly cultured cells of this model was 26–260 cfu; 1.8×10 5 viable, non‐culturable C. jejuni were introduced to day‐old chicks orally. Campylobacter jejuni was not isolated from the caeca of the chicks after incubation for 7 d. Also, passage through the allantoic fluid of embryonated eggs did not recover viable, nonculturable C. jejuni. These findings cast serious doubts on the significance of the viable, non‐culturable ‘state’ in environmental transmission of C. jejuni.