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A one‐minute oxidase test to detect Vibrio strains isolated from cultures on thiosulphate‐citrate‐bile salts‐sucrose (TCBS) medium
Author(s) -
Vila J.,
Abdalla S.,
Gonzalez J.,
Garcia C.,
Bombi J.A.,
Anta M.T. Jimenez
Publication year - 1992
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1992.tb01864.x
Subject(s) - sucrose , agar , oxidase test , vibrio , bacteria , microbiology and biotechnology , vibrionaceae , vibrio cholerae , biology , chemistry , food science , biochemistry , enzyme , genetics
J. VILA, S. ABDALLA, J. GONZALEZ, C. GARCIA, J. A. BOMBI AND M.T. JIMENEZ. 1992. Vibrio cholerae is oxidase positive, a primary characteristic used to differentiate it from Enterobacteriaceae. But false negative oxidase test results have been obtained with colonies from thiosulphate‐citrate‐bile salts‐sucrose (TCBS) agar medium. A rapid oxidase test procedure is described here. This takes 1 min, avoids false negative results and the necessity to grow the bacteria in a general‐purpose medium. The bacteria may be recovered after the test and used for further investigations.