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Sandwich capture ELISA by a murine monoclonal antibody against a genus‐specific LPS epitope for the detection of different common serotypes of salmonellas
Author(s) -
Choi Debby,
Tsang R.S.W.,
Ng M.H.
Publication year - 1992
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1992.tb01814.x
Subject(s) - serotype , monoclonal antibody , epitope , salmonella , microbiology and biotechnology , lipopolysaccharide , enterobacteriaceae , biology , antibody , virology , bacteria , chemistry , escherichia coli , immunology , gene , biochemistry , genetics
D.CHOI, R.S.W. TSANG AND M.H. NG. 1992. A sandwich capture ELISA based on a murine monoclonal antibody against a genus‐specific epitope in the outer core region of the Salmonella lipopolysaccharide is described for the detection of different common serotypes of salmonellas. Four h broth cultures of seven standard and 24 wild strains of salmonellas were all detected by the capture ELISA while overnight broth cultures of 21 non‐salmonella standard strains were all negative. The capture ELISA detected 1 ng/ml of Ra lipopolysaccharide, 10 6 /ml of a smooth wild strain of Salm. typhimurium , and 1120 cells of Salm. heidelberg after enrichment culture for 4 h.