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Isolation of salmonellas by immunomagnetic separation
Author(s) -
Vermunt A.E.M.,
Franken A.A.J.M.,
Beumer R.R.
Publication year - 1992
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1992.tb01811.x
Subject(s) - isolation (microbiology) , immunomagnetic separation , salmonella , separation (statistics) , chromatography , microbiology and biotechnology , biology , computer science , chemistry , bacteria , genetics , machine learning
A.E.M. VERMUNT, A.A.J.M. FRANKEN AND R.R. BEUMER. 1992. Magnetisable particles, coated with anti‐salmonella serum, were used to isolate Salmonella livingstone from pure cultures, mixed cultures and food samples. Beads (10 7 ) were generally incubated with 10 4 Salm. livingstone cells/ml for 60 min at room temperature. The incubation and washing medium (0.01 mol/l phosphate‐buffered saline; PBS) contained 0.1% bovine serum albumin (BSA) and 0.1% Tween 20, respectively. This method gave a recovery for Salm. livingstone of 51.9±7.8%. However, other micro‐organisms such as Aeromonas hydrophila interfered with this test because of non‐specific reactions (recovery 50.9±12.7%). These non‐specific reactions could be decreased by using 4% skim milk instead of 0.1% BSA in the incubation medium. The ratio of the recovery of Salim. livingstone relative to the recovery of Aer. hydrophila changed from 0.9 when PBS with 0.1% BSA was used, to 13.4 when PBS with 4% skim milk was used. Immunomagnetic separation of Salmonella spp. from food samples offers good prospects for concentrating salmonella cells from heterogeneous bacterial suspensions, such as enrichment broths.