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Acidic exopolysaccharide from Bradyrhizobium (Chamaecytisus proliferus)
Author(s) -
LeónBarrios M.,
GutiérrezNavarro A.M.,
PérezGaldona R.,
DíazSiverio J.,
Trujillo J.,
Corzo J.
Publication year - 1992
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1992.tb01808.x
Subject(s) - bradyrhizobium , chemistry , galactose , mannose , polysaccharide , polyacrylamide gel electrophoresis , ionic strength , polymerization , size exclusion chromatography , substituent , sugar , degree of polymerization , chromatography , biochemistry , stereochemistry , aqueous solution , polymer , organic chemistry , rhizobium , enzyme , gene
M. LEON‐BARRIOS, A.M. GUTIERREZ‐NAVARRO, R. PEREZ‐GALDONA, J. DIAZ‐SIVERIO. J. TRUJILLO AND J. CORZO. 1992. The exopolysaccharide from a strain of Bradyrhizobium isolated in the Canary Islands was studied. The polysaccharide was found to be acidic and composed of glucose, galactose, mannose and galacturonic acid in a relation 3: 1: 1: 1, respectively. Acetyl was the only acyl substituent detected. Polyacrylamide gel electrophoresis showed that it had a polymeric structure with a variable degree of polymerization. At low ionic strength it aggregated but in EDTA solutions it was resolved as a polydisperse sample by gel exclusion chromatography.