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Isolation of inclusion bodies from vegetative Clostridium perfringens: partial purification of a 47 kDa inclusion protein
Author(s) -
GarciaAlvarado J.S.,
Labbé R.G.,
Rodriguez M.A.
Publication year - 1992
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1992.tb01703.x
Subject(s) - inclusion (mineral) , isolation (microbiology) , clostridium perfringens , inclusion bodies , microbiology and biotechnology , biology , chemistry , biochemistry , bacteria , recombinant dna , genetics , gene , mineralogy
J.S. GARCIA‐ALVARADO, R.G. LABBÉ AND M.A. RODRIGUEZ. 1992. A refractile inclusion body produced by vegetative cells of Clostridium perfringens at temperatures above 40d̀C was isolated and partially characterized. The inclusion was composed of protein and could be solubilized by sodium dodecyl sulphate plus either dithiothreitol or β‐mercaptoethanol. The solubilized inclusion showed no antigenic relationship with Cl. perfringens enterotoxin. One major band with an apparent MW of 47 kDa was demonstrated after polyacrylamide gel electrophoresis of the solubilized inclusion. Both enterotoxin‐positive and enterotoxin‐negative strains produced the inclusion body. No effect on the morphology of several eucaryotic cell lines was observed when solubilized or intact inclusion was added to the cell cultures.