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A selective medium for Fusobacterium spp.
Author(s) -
Brazier J.S.,
Citron Diane M.,
Goldstein E.J.C.
Publication year - 1991
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1991.tb03798.x
Subject(s) - fastidious organism , fusobacterium , obligate anaerobe , fusobacteria , microbiology and biotechnology , fusobacterium necrophorum , josamycin , fusobacterium nucleatum , bacteroidaceae , isolation (microbiology) , biology , agar plate , bacteroides , bacteria , antibiotics , erythromycin , 16s ribosomal rna , porphyromonas gingivalis , bacteroidetes , genetics
J.S. BRAZIER, D.M. CITRON AND E.J.C. GOLDSTEIN, 1991. A new selective medium (JVN) for the isolation of Fusobacterium spp. from clinical material is described. The medium incorporates josamycin, vancomycin and norfloxacin (at 3, 4 and 1 μg/ml, respectively) as the selective agents, plus 5% defibrinated horse blood in Fastidious Anaerobe Agar Base (Lab M). This formula allowed luxuriant growth of all 82 strains (eight recognized species) of fusobacteria tested, while significantly inhibiting 51/51 (100%) strains of facultative anaerobes and 45/51 (88%) strains of other obligate anaerobes. JVN medium allowed the successful isolation of strains of Fusobacterium naviforme, F. nucleatum and F. necrophorum from the gingivae of 9/16 healthy volunteers, and strains of F. varium and F. mortiferum from faecal suspensions seeded with these organisms.