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Spontaneous occurrence of heat‐stable somatic antigen variants in Bacillus thuringiensis
Author(s) -
Mike A.,
Ohba M.,
Alzawa K.
Publication year - 1991
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1991.tb02957.x
Subject(s) - precipitin , antiserum , biology , antigen , somatic cell , bacillus thuringiensis , somatic antigen , ouchterlony double immunodiffusion , strain (injury) , population , agar , microbiology and biotechnology , genetics , gene , bacteria , demography , anatomy , sociology
Heat‐stable somatic antigen (HSSA) variants were isolated from Bacillus thuringiensis strain T84A1‐A by antiserum‐mediated selection. Substantial differences in HSSAs were clearly shown between variants and the parent strain by HSSA agglutination tests, Ouchterlony tests and precipitin‐halo‐formation (PHF) tests of extracellular HSSAs, although a ‘one way’cross‐reaction was evident. The frequency of HSSA variants in the population was < 10 ‐4 , as determined by PHF tests on antiserum‐agar plates. Heat‐stable somatic antigen variants showed no alteration in flagellar antigenic structure and in 30 phenotypic characteristics. The insecticidal activity of HSSA variants was on the level with that of the parent strain, when tested with larvae of three lepidopterous species.