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Induction and cultivation of a stable L‐form of Bacillus subtilis
Author(s) -
Allan Eunice J.
Publication year - 1991
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1991.tb02946.x
Subject(s) - bacillus subtilis , lysozyme , subculture (biology) , glycerol , microbiology and biotechnology , food science , growth medium , liquid medium , chemistry , biology , biochemistry , chromatography , bacteria , genetics
The induction of L‐forms of Bacillus subtilis from protoplasts is described. The method involved the frequent subculture of the unstable L‐form on a growth medium supplemented with lysozyme and horse serum. A stable culture, which did not revert when lysozyme and horse serum were omitted from the medium, was obtained after 13 subcultures. This culture could be grown on solid and in liquid medium by routine microbiological methods. Long‐term storage of these cells was achieved by freeze drying and maintenance in glycerol at −70°C. The cultural adaptability of the L‐form is described and discussed with respect to methods of cultivation and growth.