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Detection of Bifidobacterium species by enzymatic methods *
Author(s) -
Chevalier P.,
Roy D.,
Ward P.
Publication year - 1990
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1990.tb05227.x
Subject(s) - bifidobacterium , enzyme , actinomycetaceae , strain (injury) , lactobacillus , food science , biology , bacteria , chemistry , microbiology and biotechnology , biochemistry , fermentation , genetics , anatomy
The properties of Bifidobacterium strains of human origin were examined by three enzymic tests and the amounts of acetic and lactic acids produced were also quantified. It was evident that two strains of the American Type Culture Collection (ATCC) did not belong to the genus. Moreover, at least one strain of Bifidobacterium added to some milk preparations did not show distinctive characteristics of the genus. It was also shown that most of bifidobacteria studied produced α‐galactosidase (EC 3.2.1.22) and α‐glucosidase (EC 3.2.1.20). The presence of α‐galactosidase could afford a rapid differentiation of bifidobacteria used in some dairy products since this enzyme was not detected in Lactobacillus strains studied.