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Comparative study of cell‐immunoenzymatic methods for the estimation of IgG and IgM anti‐brucella antibodies in the diagnosis of human brucellosis
Author(s) -
Mongini Claudia,
Fernandez Teresa,
Turovetzky Arno,
Hajos Silvia E.
Publication year - 1990
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1990.tb02915.x
Subject(s) - brucella , complement fixation test , brucellosis , antibody , serology , immunoglobulin m , immunology , immunoglobulin g , direct agglutination test , virology , medicine
Two methods which employ whole cells are described and compared for the detection of human IgG and IgM anti‐brucella antibodies. Dot ELISA and ELISA were shown to be suitable for a screening diagnosis of human brucellosis. Titres of antibodies obtained by dot ELISA showed 100% coincidence for IgG and 97% for IgM, compared with agglutination and complement fixation tests; when ELISA was used 11 % positive sera were not detected. The comparison of these two methods with the conventional serological test kit indicated that both dot ELISA and ELISA were sensitive, reproducible and specific for the quantification of IgG and IgM anti‐brucella antibodies.