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The ineffectuality of potato protease inhibitor on the extracellular protease from Erwinia carotovora subsp. carotovora
Author(s) -
Heilbronn Jacqueline,
Lyon G. D.
Publication year - 1990
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1990.tb02907.x
Subject(s) - erwinia , protease , microbiology and biotechnology , biology , extracellular , chymotrypsin , pathogen , enzyme , chemistry , biochemistry , food science , trypsin , gene
Growth conditions are described for optimum production of extracellular protease in batch cultures of the soft rot pathogen Erwinia carotovora subsp. carotovora . This protease was inhibited by approximately 96% by 1 mmol/1 EDTA and by 55–6% by 10 mmol/l cysteine thereby classifying it as a metalloprotease. It was not inhibited by a chymotrypsin inhibitor extracted from potato tubers. This evidence suggests that the potato chymotrypsin inhibitor is not associated with resistance of potatoes to E. carotovora subsp. carotovora .