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Ethanol fermentation by nystatin‐resistant strains of Saccharomyces cerevisiae
Author(s) -
Grunwaldraij H.,
Margalith P.
Publication year - 1990
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1990.tb02571.x
Subject(s) - ergosterol , nystatin , fermentation , mutant , saccharomyces cerevisiae , yeast , ethanol , strain (injury) , biochemistry , chemistry , biology , food science , microbiology and biotechnology , gene , antibiotics , anatomy
Nystatin‐resistant mutants of haploid and polyploid strains of Saccharomyces cerevisiae were isolated by plating on gradient plates with increasing nystatin concentrations (60–3000 U/ml). Some of the mutants were defective in ergosterol biosynthesis, and produced zymosterol and cholestatetraenol‐like sterols. Those mutants which do not form ergosterol produce less ethanol than the parent strains. They also had lower viability during fermentation of glucose solutions (8–13% vs. 33–47%). This became more pronounced in fermentations of higher concentrations of glucose. A nystatin‐resistant but ergosterol‐forming mutant had a similar fermentation capacity to the parent strain.