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Serogroups of potato pathogenic Erwinia carotovora strains: identification by lipopolysaccharide electrophoretic patterns
Author(s) -
Murray J.,
Fixter L.M.,
Hamilton I.D.,
Perombelon M.C.M.,
Quinn C.E.,
Graham D.C.
Publication year - 1990
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1990.tb02569.x
Subject(s) - blackleg , erwinia , microbiology and biotechnology , biology , strain (injury) , serotype , bacteria , botany , genetics , anatomy , brassica
Lipopolysaccharides were purified from 51 strains of Erwinia carotovora subsp. carotovora, atroseptica and betavasculorum representing 12 different serogroups. Analysis of the lipopolysaccharides by SDS‐PAGE showed that, irrespective of the strain or serogroup from which they were extracted, the lipopolysaccharides were composed of up to 30 components. The mobility of the components decreased in a regular fashion giving a ladder‐like appearance on the gel. The relative mobilities of components of lipopolysaccharide from each serogroup was constant so that each serogroup gave an easily identifiable ladder profile. The potato pathogenic serogroups I, XVIII, XX and XXII were examined in detail. Of 20 strains received as serogroup I, 18 gave a pattern identical to an authentic serogroup I strain. The two strains which did not give the same pattern were shown by immunological tests not to be serogroup I. Five atroseptica strains of serogroup XXII gave a distinct pattern characteristic of the serogroup while atroseptica strains of serogroups XVIII (four strains) and XX (five strains) gave patterns that could not be distinguished from each other. Analysis of lipopolysaccharides by SDS‐PAGE has been shown to be an alternative to immunological tests to identify serogroups of Erwinia carotovora associated with blackleg. It is also capable of differentiating these serogroups from erwinia serogroups not normally regarded as causing blackleg. The analysis has the advantage that, irrespective of serogroup, a positive result is always obtained.

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