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Growth inhibitory and biocidal activity of some isothiazolone biocides
Author(s) -
Collier P.J.,
Ramsey A.J.,
Austin P.,
Gilbert P.
Publication year - 1990
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1990.tb01550.x
Subject(s) - biocide , chemistry , thiol , growth inhibition , bacterial growth , cysteine , glutathione , minimum inhibitory concentration , escherichia coli , dichloroacetic acid , ethidium bromide , amino acid , stereochemistry , biochemistry , bacteria , microbiology and biotechnology , dna , biology , in vitro , organic chemistry , gene , genetics , enzyme
C ollier , P.J., R amsey , A.J., A ustin , P. & G ilbert , P. 1990. Growth inhibitory and biocidal activity of some isothiazolone biocides. Journal of Applied Bacteriology 69 , 569–577. Similar patterns of growth inhibition were observed for the three biocides, benzisothiazol‐3‐one (BIT), 5‐chloro‐N‐methylisothiazol‐3‐one (CMIT) and N‐methylisothiazol‐3‐one (MIT) against Escherichia coli ATCC 8739 and Schizo‐saccharomyces pombe NCYC 1354. After periods of induced stasis, proportional to biocide concentration, growth proceeded at an inhibited rate. Extrapolation of the static periods and inhibited growth rates against biocide concentration gave minimum growth inhibitory concentration estimates of 0.1–0.5 μg/ml for CMIT, 15–20 μg/ml for BIT and 40–250 μg/ml for MIT. Patterns of growth inhibition by CMIT and induced morphological changes in inhibited cultures suggested this com‐pound to also inhibit initiation of DNA replication. Growth inhibitory activity was rapidly quenched by the addition of thiol‐containing materials such as glutathione and cysteine. The activity of CMIT was additionally quenched by the presence of the non‐thiol amino acids valine and/or histidine. These results suggest that the chlorinated isothiazolones can react with amines as well as with essential thiol groups.