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Yeast contaminants of micropropagated plant cultures
Author(s) -
Leifert C.,
Waites W.M.,
Nicholas J.R.,
KEETLEY Julia W. W.
Publication year - 1990
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1990.tb01537.x
Subject(s) - rhodotorula , yeast , acetic acid , sucrose , fermentation , biology , food science , ethanol , contamination , microbiology and biotechnology , botany , biochemistry , chemistry , ecology
L eifert , C., W aites , W.M., N icholas , J.R. & K eetley , J.W. 1990. Yeast contaminants of micropropagated plant cultures. Journal of Applied Bacteriology 69 , 471–476. Of 36 yeast strains isolated from contaminated plant cultures 78% were Candida, 20% Rhodotorula and 2% were Cryptococcus species. Strains of Candida guilliermon‐dii represented 45% of all yeasts isolated. Yeasts grew rapidly on plant growth media with a pH of between 2.5 and 6.0 and decreased the medium pH to values of between 2.0 and 3.0. Candida yeasts growing on plant medium for 28 d metabolized about 75% of the sucrose and produced fermentation products such as ethanol and acetic acid. In contrast, Rhodotorula did not produce ethanol or acetic acid and only metabolized about 20% of the sucrose. Possible sources of contamination are discussed.