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Induction of autolysis in Enterococcus faecalis S‐47 by peptide AS‐48
Author(s) -
Gálvez A.,
Valdivia E.,
MartínezBueno M.,
Maqueda. M.
Publication year - 1990
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1990.tb01531.x
Subject(s) - autolysin , autolysis (biology) , enterococcus faecalis , peptide , chemistry , proteases , microbiology and biotechnology , antibiotics , biochemistry , trypsin , divalent , enzyme , peptidoglycan , biology , escherichia coli , organic chemistry , gene
In addition to its bactericidal mode of action, the peptide antibiotic AS‐48 exhibits a bacteriolytic effect on Enterococcus faecalis S‐47 that is associated with autolysin activation. Bacteriolysis induced by the antibiotic can be modulated by addition of EDTA, divalent cations and autolysin activators (trypsin) or inhibitors (cardiolipin), suggesting that topologic regulation of the autolysins is involved in the process. In addition, inhibitors of protein and RNA synthesis interfere markedly with bacteriolysis, as do ionophores and the ATPase inhibitor DCCD, suggesting the participation of an internal messenger in autolysin activation in the presence of AS‐48.