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A simple haemolytic method for quantitation of the delta endotoxin of Bacillus thuringiensis subsp. israelensis from crude samples
Author(s) -
Majumdar M.K.,
Sikdar D.P.,
Sarma A.B.,
Majumdar S.K.
Publication year - 1990
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1990.tb01515.x
Subject(s) - bacillus thuringiensis , bacillales , coefficient of variation , bacillaceae , biology , microbiology and biotechnology , chromatography , chemistry , bacteria , genetics , bacillus subtilis
A simple haemolytic assay method for quantitative estimation of the delta endotoxin of Bacillus thuringiensis subsp. israelensis from a crude preparation has been developed. The method has several advantages over mosquito‐larvicidal methods of assay as it is inexpensive, highly sensitive and easier to run and can be used for performing a reasonably large number of assays rapidly with high precision and with a coefficient of variation that does not exceed 1.96%.