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A chemotaxonomic method for classification of asymmetric penicillia by means of cross‐partition in aqueous two‐phase systems combined with SIMCA pattern recognition analysis
Author(s) -
STRÖM GUNNAR,
BLOMQUIST GÖRAN,
SÖDERSTRÖM BENGT
Publication year - 1989
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1989.tb05116.x
Subject(s) - partition (number theory) , conidium , aqueous medium , multivariate statistics , chemistry , chromatography , food science , botany , mathematics , biology , aqueous solution , combinatorics , statistics , organic chemistry
Conidia of three strains each of three closely related Penicillium species in the P. viridicatum group were subjected to cross‐partition in aqueous polymer two‐phase systems. The cross‐point values, the percentage of conidia in the upper phases at the cross‐point and at certain pH values, together with growth‐rate measurements on different culture media were determined and statistically treated using a multivariate classification computer program (SIMCA). The results showed that the different species were well separated, corresponding with the characterization based on mycotoxin production. Moreover, the various strains grouped well within the species. The number of culture media, and thereby the number of variables used in the analysis, could be reduced without changing the separation properties.