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Comparison of dot blot with in‐situ hybridization for the detection of Neisseria gonorrhoeae in urethral exudate
Author(s) -
SCHOONE G.J.,
CORNELISSEN W.J.G.,
VEENHUIJSEN P.C.,
SCHÖNEMAN C.E.,
TERPSTRA W.J.
Publication year - 1989
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1989.tb05109.x
Subject(s) - in situ hybridization , neisseria gonorrhoeae , dot blot , in situ , microbiology and biotechnology , southern blot , dna–dna hybridization , biology , neisseriaceae , nucleic acid thermodynamics , dna , chemistry , gene expression , genetics , gene , antibiotics , organic chemistry , base sequence
Dot blot hybridization and in‐situ hybridization with DNA probes prepared from total genomic gonococcal DNA were compared with Gram staining and culturing for the detection of gonococci in urethral exudates. Fifteen of 60 patients were positive by at least one of the four methods. Gram staining and in‐situ hybridization scored best with 13 positives but culture and dot blot hybridization yielded only 10 and 9 positives respectively. The failure to detect gonococci in culture can be explained by overgrowth in one case and possibly self medication with ampicillin before culture in another. The in‐situ hybridization test is a fast and sensitive hybridization method for the detection of gonococci in urethral exudate from men.