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Influence of nutrient‐limited growth on pathogenesis‐associated outer membrane proteins of Yersinia enterocolitica
Author(s) -
Harakeh S.,
Matin A.
Publication year - 1989
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1989.tb03397.x
Subject(s) - yersinia enterocolitica , virulence , intracellular , yersinia , microbiology and biotechnology , biology , nutrient , bacterial outer membrane , bacteria , membrane protein , biochemistry , chemistry , membrane , gene , escherichia coli , genetics , ecology
From studies based on batch culture, it has been postulated that the expression of the virulence‐associated proteins of Yersinia spp. is controlled by temperature and Ca 2+ , such that these proteins are synthesized only at the higher temperature (37°C) and calcium‐scarce conditions of the intracellular environment. It was found, however, that in Yersinia enterocolitica one of these proteins (140 kDa) is not synthesized at submaximal growth rates under any of the relevant conditions, and that another of the implicated proteins (34 kDa), is synthesized even at 28°C during nutrient‐limited growth. Thus, temperature and Ca 2+ influence the synthesis of these proteins differently under growth conditions that better approximate the natural environments than do batch cultures.