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16S Ribosomal ribonucleic acid sequence analyses of lactococci and related taxa. Description of Vagococcus fluvialis gen. nov., sp. nov.
Author(s) -
Collins M.D.,
Ash C.,
Farrow J.A.E.,
Wallbanks S.,
Williams A.M.
Publication year - 1989
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1989.tb02516.x
Subject(s) - biology , enterococcus , microbiology and biotechnology , phylogenetic tree , lactococcus , ribosomal rna , 16s ribosomal rna , phylogenetics , ribosomal dna , streptococcus , genetics , bacteria , gene , lactococcus lactis , lactic acid , antibiotics
The phylogenetic status of members of the genus Lactococcus and some motile strains which react with Lancefield group N antiserum was examined by reverse transcriptase sequencing of 16S ribosomal ribonucleic acid. In agreement with earlier nucleic acid hybridization and immunological studies of superoxide dismutase the 16S sequence data clearly demonstrate that the lactococci represent a distinct phylogenetic group equivalent in rank to the genera Enterococcus and Streptococcus. The motile group N strains from chicken faeces and river water, however, were found to be phylogenetically unrelated to lactococci but displayed a closer, albeit loose, association with members of the genus Enterococcus. On the basis of the present sequence data and earlier chemotaxonomic studies it is proposed that the motile group N strains be classified in a new genus Vagococcus , as Vagococcus fluvialis sp. nov. The type strain of V. fluvialis is NCDO 2497.