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Detection of β‐galactofuranosidase production by Penicillium and Aspergillus species using 4‐nitrophenyl β‐D‐galactofuranoside
Author(s) -
COUSIN M.A.,
NOTERMANS S.,
HOOGERHOUT P.,
VAN BOOM J.H.
Publication year - 1989
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1989.tb02484.x
Subject(s) - penicillium , aspergillus , microbiology and biotechnology , penicillium digitatum , extracellular , extracellular polysaccharide , chemistry , biology , food science , polysaccharide , biochemistry , antifungal
An assay was developed for detecting β‐galactofuranosidase produced by Penicillium and Aspergillus spp. The substrate for the assay, 4‐nitrophenyl β‐D‐galactofura‐noside, was synthesized from penta‐O‐acetyl‐β‐D‐galactofuranose and 4‐nitrophenol by a tin chloride catalyzed reaction followed by O‐deacetylation. Aspergillus spp. produced only small quantities of β‐galactofuranosidase during 30 d at 25°C. Only the biverticillate Penicillium spp. ( P. funiculosum, P. islandicum, P. rubrum and P. tardum ) produced substantial β‐galactofuranosidase after 1–4 weeks at 25°C. No extracellular antigens of these four Penicillium spp. could be detected in culture filtrates by the sandwich ELISA technique when antibodies to the extracellular β‐galactofuranoside‐containing polysaccharide antigen of P. digitatum was used. Antigens to all other Penicillium and Aspergillus spp. were easily detected in their culture filtrates.