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Investigation of a simple amperometric electrode system to rapidly quantify and detect bacteria in foods
Author(s) -
Patchett R.A.,
Kelly Alison F.,
Kroll R.G.
Publication year - 1989
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1989.tb02453.x
Subject(s) - thionine , contamination , bacteria , amperometry , food science , chemistry , incubation , chromatography , electrode , environmental chemistry , microbiology and biotechnology , biology , electrochemistry , biochemistry , ecology , genetics
A two electrode system mounted as a single probe was developed to measure electrochemically the rate of reduction of a redox mediator (thionine) by bacteria. The system gave a rapid (2 min) bacterial‐dependent current above 2.5×10 5 cfu/ml with pure cultures of bacteria, but when applied to the measurement of the bacterial contamination in samples of meat and milk it was unable to detect or quantify the contamination reliably. Incubation of samples for a few hours before examination enabled the system to detect bacteria in excess of 10 6 cfu/ml.