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Counter‐immunoelectrophoresis for the detection of Brucella antigen and antibodies in the diagnosis of brucellosis in buffaloes
Author(s) -
Chand Puran,
Khanna R.N.S.,
Sadana J.R.
Publication year - 1988
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1988.tb05101.x
Subject(s) - immunoelectrophoresis , brucellosis , complement fixation test , brucella , antigen , antibody , agglutination (biology) , counterimmunoelectrophoresis , direct agglutination test , latex fixation test , hemagglutination , immunodiffusion , brucellaceae , microbiology and biotechnology , biology , medicine , immunology , serology , brucella melitensis
Counter‐immunoelectrophoresis was employed for the detection of Brucella antigen in stomach contents of aborted buffalo fetuses and antibody in aborted as well as apparently healthy in contact buffaloes. Five of 16 aborted cases were serologically positive for brucellosis but isolation of Brucella abortus was successful in only two cases. By counter‐immunoelectrophoresis, Brucella antigen was detected in the fetal stomach contents of four serologically positive cases. Of the 68 serum samples from in contact healthy buffaloes, 10 were positive with counter‐immunoelectrophoresis: more than were detected by tube agglutination, Rose Bengal plate agglutination, complement fixation and agar gel precipitation test.