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Azelaic acid: its uptake and mode of action in Staphylococcus epidermidis NCTC 11047
Author(s) -
Bojar R.A.,
Holland K.T.,
Leeming J.P.,
Cunliffe W.J.
Publication year - 1988
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1988.tb02441.x
Subject(s) - azelaic acid , staphylococcus epidermidis , staphylococcus aureus , in vitro , mode of action , biochemistry , chemistry , bacteria , biology , microbiology and biotechnology , genetics
In vitro tests using Staphylococcus epidermidis as a model have shown that at pH 5.6 the micro‐organisms are sensitive to azelaic acid, whilst at pH 6.0 and 7.0 the cells become progressively resistant, especially with nutrients present. In a simple defined medium the growth rate was reduced at 1 mmol/l and growth inhibited at 25 mmol/l. The uptake of azelaic acid was pH dependant, higher transport at lower pH values, and required viable cells. Azelaic acid, 457 μmol/l gave 50% inhibition of protein synthesis and this mechanism could account for the bactericidal and bacteristatic effects. DNA and RNA were affected slightly by 100 mmol/l azelaic acid, and respiration by 500 mmol/l