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Production of antibody to lipopolysaccharide (LPS) after immunization with a LPS‐polymyxin B‐agarose immunogen
Author(s) -
Ryan Lisa K.,
Karol Meryl H.
Publication year - 1988
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1988.tb02440.x
Subject(s) - immunogen , antibody , lipopolysaccharide , polymyxin b , immunization , antigen , microbiology and biotechnology , polymyxin , adjuvant , agarose , biology , immunology , monoclonal antibody , antibiotics
A method was devised to produce antibodies to lipopolysaccharide (LPS) in guineapigs following a single immunization, The antigen was prepared by mixing polymyxin B‐agarose with LPS from Escherichia coli O55:B5. Use of the agarose support allowed purification of the complex by simple washing procedures. Twenty‐nine days after a single injection of the immunogen mixed with Freund complete adjuvant all animals demonstrated antibody to the LPS portion of the complex. No antibodies were detected to the polymyxin B component. Typical titres of LPS as measured by ELISA were 2 11 . After, a booster immunization, titres of LPS antibody were further inceased and a greater avidity was noted. In contrast to other methods which have been employed for production of antibody to LPS, use of the polymyxin B‐agarose complex has the following advantages: ease of antigen preparation, ready purification of the complex, potent immunostimulation, and under the conditions employed here, LPS‐specific antibody production, without accompanying antibody to polymyxin B.