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Polygalacturonase biosynthesis by Lactobacillus plantarum : effect of cultural conditions on enzyme production
Author(s) -
Sakellaris G.,
Nikolaropoulos S.,
Evangelopoulos A.E.
Publication year - 1988
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1988.tb01908.x
Subject(s) - pectinase , lactobacillus plantarum , enzyme , biosynthesis , pectin , chemistry , inducer , enzyme assay , biochemistry , extracellular , food science , bacteria , biology , lactic acid , gene , genetics
Lactobacillus plantarum was found to produce extracellular polygalacturonase (EC 3.2.1.15.). Maximum enzyme production was obtained in a medium containing 0.5% glucose and 1.5% low methyl‐pectin as inducer at 27°C at an initial pH of 6.8. Enzyme production was strongly inhibited by 5 μmol/l NiCl 2 , 5 μmol/l CoCl 2 , 5 μmol/l CuSO 4 , and 10 μmol/l ZnCl 2 . MnSO 4 and MgSO 4 at 200 μmol/l and 50 μmol/l respectively seemed to enhance enzyme biosynthesis. The optimal pH and temperature for enzyme activity were 4.5 and 30°C respectively. Enzyme production in batch culture accompanied growth.